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KMID : 0364219690120030069
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Korean Journal of Zoology
1969 Volume.12 No. 3 p.69 ~ p.76
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Analysis of Phagocytosis and Birefringence in the Peritoneal Cells of the Rat, with Special Regard to the Mast Cells
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Oh Yung-Keun
Shin Hyun-Sam
Bang Hyuck
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Abstract
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The phagocytic petencies and birefringences of peritoneal mast cells of rats treated with particular dyes (neutral red, toluidine blue, pyronin, methylene blue, alcian blue, trypan blue, carmine, orange G, aniline blue, Janus green B, and India ink) were analyzed by means of phase contrast microscopy and polarizing microscopy. In addition, cytomorphic effects of the dyes on the peritoneal mast cells were also discussed. Phagocytic activities or ingestion of the dye particles were not observed in most cases, except for the India ink group. Hardly a macrophage appeared without some dark particles which were ingested or phagocytosed. Trypan blue and aniline blue produced very weak birefringence in the cytoplasm of mast cells but the rest did not produce even in the acid medium (neutral red and toluidine blue). The short and slender ectoplasmic processes of the mast cells and the leucocytes were also found in certain groups. The cytomorphic effects of the dyes on the mast cell were slight and variable.
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